FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3029_d3.352000000b7a88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3029_d3.352000000b7a88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847806
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG508510.3208709142451643No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC384690.24274022536621156No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC259020.16344218246992676No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC232900.14696040574954033No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA203700.12853514234083885No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC195720.12349974501202247No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT187370.1182308768797397No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT180340.11379493161387765No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA179940.1135425307452653No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA160920.1015408694427481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT112700.045.7657361
AACCGTG117500.043.484172
ACAACCG53600.035.469562
ACCGTGT146100.035.1019943
CCGTGTT158050.032.416364
CGTGTTC173250.029.5351035
TTCACTA203700.025.2817349
GTTCACT213850.024.059498
TGTTCAC217350.023.8032077
GTGTTCA231050.022.761136
ACAGACT128950.021.599162
AGACTCA147200.019.8889964
CACAACC100550.019.7146281
AACTCAC215700.019.5375235
ACTCACC224450.019.0067946
GAACTCA248300.017.4756774
AGAACTC253200.017.0068973
CACAGAC185650.016.0804921
CAGACTC186750.015.7794413
TCACCAT291150.014.7509128
Produced by FastQC (version 0.11.2)