FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3024_d3.352000000b7b1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3024_d3.352000000b7b1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13956914
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG445870.3194617377451778No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC330520.23681452791068283No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC217350.15572926794562178No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC205130.14697375078760247No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC168240.1205424064374116No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA165290.11842875867831527No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT164320.11773376263549379No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT159830.11451671909707262No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA156160.11188719798660363No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC147540.10571104758544761No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA142490.10209276921818104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT100500.043.232221
AACCGTG102250.042.3926352
ACCGTGT127900.033.8537453
ACAACCG49150.033.265482
CCGTGTT142600.030.6960054
CGTGTTC154000.028.6616575
TTCACTA176800.024.7752259
TGTTCAC187750.023.8363597
GTTCACT187200.023.6272288
GTGTTCA196800.022.9324446
ACAGACT110250.021.167122
AGACTCA122150.020.427144
CACAACC85850.020.2106361
AACTCAC179050.019.264985
ACTCACC183750.018.5113246
GAACTCA207950.017.1641674
AGAACTC217200.016.1931293
CAGACTC158400.016.1730693
CACAGAC155050.016.065231
CACTAGC162800.015.11625910-11
Produced by FastQC (version 0.11.2)