FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3010_d5.352000000b7ae5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3010_d5.352000000b7ae5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13758879
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG406520.295460117063316No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC309940.22526544495376405No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC211730.1538860833066415No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC182150.13238723881502265No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC162470.1180837479565014No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA162100.11781483069950684No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT149800.10887514891293107No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT143590.1043616998158062No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA139160.10114196076584436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT94300.043.9071161
AACCGTG99450.041.625672
ACAACCG45600.035.228952
ACCGTGT126000.032.8545463
CCGTGTT141600.029.669814
CGTGTTC150950.027.8236335
TTCACTA167300.024.8753789
GTTCACT177150.023.4922458
TGTTCAC183250.022.8398597
GTGTTCA193700.021.9256276
ACAGACT107250.020.296262
AGACTCA113650.020.0305024
CACAACC87750.019.0146561
AACTCAC176250.018.5731165
ACTCACC179850.018.2520546
GAACTCA205850.016.3689614
AGAACTC209000.016.0548523
CACAGAC157650.015.4384771
CAGACTC156700.015.1652263
CACTAGC157450.014.48305210-11
Produced by FastQC (version 0.11.2)