FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3004_d8.352000000b7b6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3004_d8.352000000b7b6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16777936
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG780140.4649797209859425No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC445660.265622660618088No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT307450.18324661627032074No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA295940.17638641606452665No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT292060.1740738550915917No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC287730.17149308472746588No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC279770.1667487586077334No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA275100.1639653411480411No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC269250.1604786190625593No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC232320.13846756835882554No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA229660.13688215284645264No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA215680.1285497810934551No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG198850.11851874986291519No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG195790.11669492600281703No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC192190.11454925087328978No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC186100.11091948377917284No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC175790.1047745086165545No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC175220.10443477672104602No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG173770.10357054646054199No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC171260.10207453407856604No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG168210.10025667042716101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT149600.050.430071
AACCGTG163800.045.9927752
ACAACCG68650.040.6416972
ACCGTGT205350.036.6867183
CCGTGTT227650.033.195894
CGTGTTC242850.031.1269575
TGTTCAC289600.026.2970587
TTCACTA289900.026.0568059
GTGTTCA293600.026.0186866
GTTCACT297700.025.4698458
CACAACC115300.025.1916221
ACAGACT129500.022.719312
AGACTCA145550.021.3560224
AACTCAC226850.020.3578575
ACTCACC234350.019.8460046
GAACTCA257700.018.36964
AGAACTC262200.017.6200793
CACAGAC178450.017.4755761
CAGACTC184050.016.9669513
CGCCCTT54450.015.2786291
Produced by FastQC (version 0.11.2)