FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n02_3002_d3.352000000b7af2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n02_3002_d3.352000000b7af2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14399512
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG468110.32508740573986117No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC347380.2412442866119352No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC230480.16006097984431694No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC207390.14402571420475918No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC182090.12645567433118568No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA177110.12299722379480638No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT168090.11673312262248889No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA167790.11652478222873108No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT166460.11560113981640488No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC149710.10396880116492838No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA148460.1031007161909376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT100450.044.105911
AACCGTG104400.042.3857272
ACAACCG47500.035.8228262
ACCGTGT133850.033.1664473
CCGTGTT146650.030.4321634
CGTGTTC157200.028.5620735
TTCACTA181750.024.6237269
GTTCACT190550.023.685938
TGTTCAC194200.023.5098537
GTGTTCA199750.022.9272756
CACAACC85300.021.0098571
AGACTCA124550.020.1865124
ACAGACT116950.020.1582072
AACTCAC187250.020.0200735
ACTCACC192400.019.0622626
GAACTCA213900.017.5980174
AGAACTC218400.017.2144973
CACAGAC163200.015.9912731
CAGACTC168400.015.7493463
CACTAGC171750.014.49478710-11
Produced by FastQC (version 0.11.2)