FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3033_d5.351000000b7a55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3033_d5.351000000b7a55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393414
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG738390.513005462081477No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC432030.3001581139818531No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC340350.2364623153339437No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC316120.21962822718779576No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT273530.19003830501922617No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC256380.17812313326080942No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA250090.17375307901238718No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA235930.16391524623692474No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT235540.16364428897827854No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA199450.13857032112047912No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC166680.1158029637721808No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC161420.11214851459146523No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA150420.10450613037323876No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC147180.10225510083987023No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG146400.10171318632257781No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC144610.10046956198161187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT143150.049.6928981
AACCGTG155150.045.9315722
ACAACCG67800.043.4444052
ACCGTGT193550.036.940913
CCGTGTT206700.034.6363834
CGTGTTC223450.031.9548575
CACAACC102750.029.5979791
ACAGACT123500.027.9665762
TGTTCAC260950.027.6666817
TTCACTA265000.027.1183839
GTGTTCA269150.026.8644856
GTTCACT269700.026.698638
AGACTCA144850.024.630954
CACAGAC165050.021.938321
CAGACTC176650.020.4123233
AACTCAC225800.019.0827795
ACTCACC233150.018.6645836
GAACTCA256150.017.3225694
AGAACTC254600.017.1669733
CGCCCTT42400.016.3625471
Produced by FastQC (version 0.11.2)