FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3029_d3.351000000b7a8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3029_d3.351000000b7a8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847806
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG525020.331288760097139No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC379210.23928233346622238No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC258150.1628932105806949No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC231070.14580567177563886No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC199660.1259858935678541No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT199500.12588493322040917No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA196070.12372059577205828No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA186580.1177323851642303No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT179060.11298724883431813No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA159690.10076473677176512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT112500.047.7317161
AACCGTG120200.044.6239282
ACCGTGT153600.034.982083
ACAACCG53350.032.9492572
CCGTGTT168500.032.2266434
CGTGTTC179400.030.2419415
TTCACTA212950.025.3600529
TGTTCAC223200.024.5997667
GTTCACT224850.024.229138
GTGTTCA229550.024.0902716
AGACTCA140450.021.1410714
ACAGACT132450.020.840232
AACTCAC206300.019.367025
CACAACC99450.018.8744131
ACTCACC213800.018.643196
GAACTCA239150.017.0048224
AGAACTC246850.016.3783683
CAGACTC185050.015.9945913
CACAGAC183950.015.8616991
CACTAGC200800.014.86650710-11
Produced by FastQC (version 0.11.2)