FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3024_d3.351000000b7b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3024_d3.351000000b7b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13956914
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG473220.3390577601896809No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC343500.2461145780507066No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC226070.16197706742335735No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC207750.1488509565939863No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC179590.12867457662918894No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT176900.12674721646919943No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA172670.12371646052988505No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA167870.1202773048540673No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT164110.11758329957467675No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC149730.10728016236253946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT102900.043.0856481
AACCGTG103350.042.6602022
ACAACCG47750.035.42112
ACCGTGT130800.033.779353
CCGTGTT143700.030.945114
CGTGTTC155700.028.6514645
TTCACTA179800.024.8579649
TGTTCAC191650.024.1388057
GTTCACT189950.023.6296968
GTGTTCA200900.023.0092666
ACAGACT108450.021.0717682
CACAACC87200.020.2718221
AGACTCA124150.019.9754584
AACTCAC183700.019.9136775
ACTCACC184900.019.8615196
GAACTCA211100.017.8692054
AGAACTC213400.017.4763743
CAGACTC158200.016.2467633
CACAGAC154800.016.2386911
CACTAGC169100.014.84454810-11
Produced by FastQC (version 0.11.2)