FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3010_d5.351000000b7ae8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3010_d5.351000000b7ae8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13758879
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG420740.3057952613726743No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC314470.22855786434345413No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC218500.15880654230624458No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC179820.13069378689935424No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC169480.12317863977145231No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA165970.12062755984699045No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT156350.11363571116513199No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT149740.10883154070909411No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA145750.10593159515393659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT97850.042.6849751
AACCGTG101900.040.840712
ACAACCG48750.038.9806142
ACCGTGT132750.031.3851093
CCGTGTT144100.029.0117654
CGTGTTC156000.026.9508555
TTCACTA175500.023.8429249
GTTCACT182450.023.0127818
TGTTCAC188900.022.4533027
CACAACC88150.022.262541
GTGTTCA195250.021.800726
ACAGACT109250.020.0032122
AGACTCA117100.019.6761574
AACTCAC174700.018.7361415
ACTCACC178900.018.2697246
GAACTCA197100.016.9925274
AGAACTC205650.016.1475663
CACAGAC153000.015.1246221
CAGACTC157750.014.5758743
CACTAGC164500.014.06124510-11
Produced by FastQC (version 0.11.2)