FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3004_d8.351000000b7b60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3004_d8.351000000b7b60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16777936
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG809880.48270538163931487No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC459830.2740682763362549No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT311450.18563069974757324No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA305970.18236450538373733No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT305450.1820545745316945No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC297550.17734600966412079No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC282640.16845933850266206No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC275000.16390573906110978No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA270980.16150973516647102No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC233430.13912915152376312No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA223040.1329364946915997No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA220680.13152988544002076No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG202350.12060482290551114No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC196370.11704061810701866No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG195020.11623598993344593No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC185410.11050822937934678No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC179310.1068725020765367No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC178280.1062586005811442No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG177030.10551357449450277No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC175790.1047745086165545No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG175580.10464934423399874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT153250.052.3418541
AACCGTG163800.048.9608542
ACAACCG65750.042.3440362
ACCGTGT206600.038.7942543
CCGTGTT228550.035.1307034
CGTGTTC247100.032.5894475
TGTTCAC293700.027.6541887
GTGTTCA297850.027.339676
TTCACTA293650.027.2545249
GTTCACT303850.026.5115648
CACAACC111250.025.5433751
ACAGACT128100.023.4400392
AGACTCA141200.022.61084
AACTCAC234250.019.9772955
ACTCACC238450.019.3664046
CACAGAC177250.018.6058861
GAACTCA264350.017.702654
CAGACTC181050.017.6341043
AGAACTC265600.017.4226233
ATTAGGC69400.016.6351555
Produced by FastQC (version 0.11.2)