FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l02n01_3002_d3.351000000b7af5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l02n01_3002_d3.351000000b7af5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14399512
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG486800.3380670122709714No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC346880.24089705262233885No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC236190.16402639200550684No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC208570.14484518642020647No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC191890.13326146052727342No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT185880.12908770797232572No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA175840.12211524946123173No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA173050.12017768379928431No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT165200.11472611016262219No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC153280.10644805185064604No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA146940.1020451248625648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT106350.043.3414231
AACCGTG114500.040.5380632
ACAACCG52300.035.699722
ACCGTGT146800.031.7475953
CCGTGTT159200.029.3641134
CGTGTTC169650.027.639185
TTCACTA193500.024.0556149
TGTTCAC210050.022.7707947
GTTCACT210600.022.2602548
GTGTTCA214000.022.110956
CACAACC92150.021.1425531
ACAGACT116550.020.5035762
AGACTCA125800.019.9396784
AACTCAC191400.018.417215
ACTCACC199550.017.5221716
GAACTCA221600.016.4862334
AGAACTC227850.015.5754093
CACAGAC165150.015.3649661
CAGACTC166400.015.2459963
CACTAGC187550.014.06819310-11
Produced by FastQC (version 0.11.2)