FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l01n02_3036_d3.352000000b7abe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n02_3036_d3.352000000b7abe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13905336
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG396420.2850848048547694No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC262310.18863981424109422No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC169680.12202509885413772No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC153460.11036051196461559No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT147000.10571481336373319No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC146580.10541277103983679No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA141140.10150060379698843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT91950.042.992051
AACCGTG95500.041.1423452
ACAACCG46300.033.3494952
ACCGTGT123950.031.8883763
CCGTGTT136450.028.8980164
CGTGTTC148250.026.9185185
TTCACTA164250.023.7472729
GTTCACT171650.023.000288
TGTTCAC181550.022.0600877
GTGTTCA191250.021.3135386
CACAACC82650.019.200841
AACTCAC152800.018.1885935
AGACTCA107600.017.8815944
ACTCACC160600.017.1870086
ACAGACT104200.016.1862982
GAACTCA181450.016.0758824
AGAACTC185750.015.4988493
CACTAGC157700.013.81268510-11
TCACTAG159250.012.67885310-11
TCACCAT227750.012.6203958
Produced by FastQC (version 0.11.2)