FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l01n02_3015_d5.352000000b7b2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n02_3015_d5.352000000b7b2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16424877
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG619560.3772083042083055No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC480230.2923796628735789No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC367300.22362420126494706No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA281040.17110630417506323No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC271900.16554157452746832No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC271600.16535892475785358No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT218700.13315168204912586No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT210110.12792181031249122No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA193940.11807698773025818No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC187980.11444834564057922No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA182190.11092320508701527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT141300.044.1165731
AACCGTG147750.042.222
ACAACCG69250.041.437942
ACCGTGT183400.034.0108343
CCGTGTT197750.031.6394634
CGTGTTC215100.029.2421115
ACAGACT142900.026.9963322
CACAACC112850.026.0193821
TTCACTA240850.025.9779919
TGTTCAC248650.025.4305657
GTTCACT253600.024.7468558
GTGTTCA260650.024.2779276
AGACTCA163350.024.1098884
CACAGAC194000.020.817441
CAGACTC207900.019.4915523
AACTCAC253100.019.2582325
ACTCACC260900.018.9375746
GAACTCA282650.017.782654
AGAACTC289950.017.0560913
CTCAGAA245250.015.6907037
Produced by FastQC (version 0.11.2)