FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l01n02_3006_d5.352000000b7a2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n02_3006_d5.352000000b7a2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14138776
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG273660.19355282239424404No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC202590.14328680219560733No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC157670.11151601807681231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT76250.035.7673031
AACCGTG77200.035.3864562
ACAACCG42850.028.4950072
ACCGTGT103350.026.7987063
CCGTGTT113800.024.2963524
CGTGTTC124350.022.2353365
TTCACTA132300.020.7560779
GTTCACT145800.018.9971458
TGTTCAC158100.017.849747
GTGTTCA170300.016.9892926
AACTCAC139050.016.4680655
ACTCACC139850.016.2721796
AGACTCA113700.016.0446834
CACAACC86900.015.145171
ACAGACT116500.015.1298462
GAACTCA169850.013.5934934
AGAACTC176500.012.8389463
CACTAGC118550.012.76374910-11
CAGACTC156650.011.8880723
CACAGAC164100.011.8421041
Produced by FastQC (version 0.11.2)