FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l01n01_3036_d3.351000000b7ab1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n01_3036_d3.351000000b7ab1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13905336
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG424310.30514185345826955No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC256890.18474202996605046No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC169040.12156484388439086No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT167320.12032790865319615No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC162760.11704859199374974No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA146910.10565009000861252No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC146480.10534085620081385No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA142320.10234919889745922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT95400.043.7424431
AACCGTG100900.041.3421482
ACCGTGT127500.032.716693
ACAACCG46850.031.437052
CCGTGTT139850.029.8954584
CGTGTTC151650.027.6628575
TTCACTA169850.024.4683199
GTTCACT181650.023.0618848
TGTTCAC188000.022.6618947
GTGTTCA199850.021.419016
CACAACC83400.018.692381
AACTCAC157450.018.285585
ACAGACT109050.018.0370032
AGACTCA115400.017.9500524
CGTATGC35450.017.8316781
ACTCACC161750.017.8288426
TATGCCG36550.017.0282083
GAACTCA188550.015.899664
AGAACTC187400.015.769063
CACTAGC166300.013.75199410-11
Produced by FastQC (version 0.11.2)