FastQCFastQC Report
Wed 10 Jan 2018
H5JWNBCX2_l01n01_3015_d5.351000000b7b2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n01_3015_d5.351000000b7b2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16424877
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG646980.39390249315109027No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC477910.2909671713218918No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC365990.22282663060429614No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC285400.1737608141601304No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA282110.17175775502002238No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC269580.1641290829757812No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT236530.14400716668989363No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT209160.12734341937537796No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA201570.12272238020412574No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC186350.11345594855900595No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA179480.10927326883482902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT145050.045.086241
AACCGTG150050.043.441552
ACAACCG70100.042.0204662
ACCGTGT192600.034.0413933
CCGTGTT208550.031.323994
CGTGTTC225750.029.1896785
CACAACC111300.027.1584661
ACAGACT138750.026.9481492
TTCACTA256950.025.6012659
TGTTCAC262550.025.4712897
GTTCACT261250.025.1980678
GTGTTCA272350.024.7359246
AGACTCA160200.023.6956624
CACAGAC195000.019.9614721
CAGACTC203500.019.5409473
AACTCAC253300.019.0750795
ACTCACC262350.018.0910456
GAACTCA279600.017.4509014
AGAACTC288600.016.8573073
CTCAGAA241750.015.3442217
Produced by FastQC (version 0.11.2)