FastQCFastQC Report
Tue 9 Jan 2018
H5JWNBCX2_l01n01_3006_d5.351000000b7a2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JWNBCX2_l01n01_3006_d5.351000000b7a2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14138776
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG283530.20053362469283054No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC201400.1424451451808841No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC155190.10976197656713707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT76700.034.885741
AACCGTG77950.033.7679022
ACAACCG41400.027.3141082
ACCGTGT99900.026.87143
CCGTGTT114350.023.517314
CGTGTTC123550.022.0347675
TTCACTA131550.020.2913049
GTTCACT143750.018.7013558
TGTTCAC154600.018.0033197
GTGTTCA168600.016.5697656
TATGCCG38300.016.1269383
CGTATGC39350.015.94278051
AACTCAC134200.015.6482715
AGACTCA119000.015.5313794
ACTCACC136700.015.292586
CACAACC85800.014.9004651
ACAGACT114150.014.5264642
GAACTCA165650.013.2225994
CCGGTAT12250.012.803031
CACTAGC119250.012.54632910-11
Produced by FastQC (version 0.11.2)