FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5316153
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2337724.397390368561627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC61300.028.1488755
CGTATCA62700.027.97524356
CGCCGTA63700.027.93723953
GTATCAT62350.027.52353557
GCCGTAT65300.027.22171454
TCATTAA68550.026.93968860
TCGCCGT65350.026.89522452
CGAGATC7950.025.4414255
TATCATT69700.025.3738558
CTCGGTG68450.025.26547844
TCTCGGT68700.025.17235243
GATCTCG69250.024.4689541
AGATCTC71600.023.85905840
TCGGTGG77900.023.05573545
TAGATCT75400.022.98027839
AAGATCG41300.022.284756
CATTAAA89600.021.63052461
CGCACAG2600.021.5214481
GTGGTCG87100.020.90132548
ATCATTA89050.020.69355259
Produced by FastQC (version 0.11.5)