FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9NEW-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9NEW-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7590019
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77820.1025293876075936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAG7950.022.082961
CGGCAAG22300.020.6262761
CGCGCAG4150.019.459611
CGCGAAT8900.018.5422421
CGGGGAG7300.017.7964441
CGGGAAG71550.017.2737831
TGCCCCG6700.017.23547743
CGCGGAG5300.017.2247091
TAGTCCG21700.017.09347211
TTAACCG43300.016.89194958
CGCCAAA30250.016.83051
GTCCGCA22050.016.82214713
CGCCAAG17750.016.814151
CGGGGTG9200.016.792651
GCCCCGG7000.016.49681544
CGGGCTA5450.016.1063791
CGGCAAA59600.016.0242251
CGCGGCT8400.015.83330268
ACCGTAG47400.015.65294561
CCGTAGA48050.015.3682662
Produced by FastQC (version 0.11.5)