FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9NEW-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9NEW-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7594059
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105430.13883221081110905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAG7150.019.6459141
GTCGGCG18300.019.31417331
CGGCAAG22100.018.7502941
TGTCGGC19400.018.39942630
CGGCGGC20000.018.37248833
CGGGAAG59900.017.5292241
CGGGGTG8500.017.3519671
TCGGCGG21000.016.83103232
CGCCAAA29000.016.7108821
GCCCCGG6100.016.63630544
TGCCCCG6100.016.63630543
CGCGAAG11550.016.1143281
CGCCAAG17950.016.0423411
CGGCAAA57000.015.710271
GTGGGAC24250.015.1517719
CCCGGGT6300.014.99725846
CGGCCAG5950.014.7550741
TAGTCCG19150.014.61864211
ATGTCGG25550.014.38140629
CCCCGGG6750.013.99744145
Produced by FastQC (version 0.11.5)