FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6393895
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124040.19399755548065772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA212700.037.0187153
CCGTATC209650.036.9062755
TCGCCGT215100.036.52432652
GTCGCCG217150.036.51794451
GGTCGCC217500.036.49050550
GCCGTAT216600.036.303754
CGTATCA214950.036.09367856
CTCGGTG211750.035.66351344
TGGTCGC220950.035.6353649
CGAGATC31500.035.4621475
TCTCGGT213400.035.17458343
GATCTCG213050.035.1680441
ATCTCGG214600.035.1586442
GTGGTCG224700.035.04064648
GTATCAT219200.034.67518657
GGTGGTC229100.034.3829547
AGATCTC220500.034.12266540
TCGGTGG230700.033.56834445
TAGATCT232500.032.75284239
CGGTGGT241750.032.56957646
Produced by FastQC (version 0.11.5)