FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7291443
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156140.21414142577813475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA214950.021.76873861
TCATTAA215650.021.51881860
CGTATCA215900.021.4116856
CCGTATC216400.021.29752355
GTATCAT218100.020.8908457
CGCGCAG4400.020.7451821
CGCCGTA228100.020.41960753
CGAGATC24700.020.2722435
GCCGTAT232150.020.10887354
TCGCCGT232650.019.89992352
ATCATTA234750.019.73816559
CGGCAAG21150.019.5870131
CGGGCAG7400.019.4513051
GTCGCCG240600.019.37328351
CGGGCTG4600.019.0800191
TATCATT240800.019.0678558
GGTCGCC245750.018.99550850
TGGTCGC253200.018.39513649
CTCGGTG246100.018.31453344
CGCGAAG9700.018.0965121
Produced by FastQC (version 0.11.5)