FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7067704
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170520.24126647069543378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC241350.028.6955955
CGTATCA242250.028.69010256
CGCCGTA249850.027.91544753
CATTAAA253150.027.77430761
GTATCAT246550.027.73531757
GCCGTAT253350.027.59886454
TCGCCGT254050.027.41203952
TCATTAA253050.027.38342760
GTCGCCG259450.027.17870551
GGTCGCC263900.026.86475650
CGAGATC27550.026.5649345
TGGTCGC267500.026.4247249
CTCGGTG259400.026.30574244
ATCATTA266500.026.02785959
TCTCGGT263850.025.80921243
ATCTCGG264350.025.78741642
GATCTCG263300.025.75734741
GTGGTCG275300.025.70146248
TATCATT271150.025.42661958
AGATCTC274900.024.84867740
Produced by FastQC (version 0.11.5)