FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934291
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160280.231141150551657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTG15500.022.657271
CGGGCAG6050.021.4775961
CGCGAAG9500.021.4409351
CGAGATC21500.020.0319185
CGCGCAG3550.018.7959611
CGGCAAG19300.018.5601791
CGGGAAG56850.018.347011
CATTAAA200300.018.24039561
TCATTAA202250.017.76986160
CGGCCTA3400.017.5593851
CGGGGTA9450.017.4664781
CGCGAAT8900.017.362091
CGGGCTG3650.017.3188461
CGCCAAG14450.017.0125541
CGTATCA214700.016.72288356
GTATCAT213600.016.62879257
CCGTATC216750.016.54857655
ATCATTA218050.016.43410359
TAGTCCG9800.016.42607911
CGCGAAA11350.016.3990751
Produced by FastQC (version 0.11.5)