FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900668
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119350.20226523505474295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG8500.022.7241081
CGGGCTA4500.021.851871
CCGGGAG3250.019.4505631
CGTATCA132200.019.43037856
CCGTATC134000.019.1954955
CGGGAAG38100.019.0804421
CGCGGAG4450.018.9406241
CGGGCTG4300.018.784621
CATTAAA138850.018.65212261
CGAGATC18250.018.6104745
CGCCGTA140300.018.50783353
GTATCAT138400.018.38278657
CGCGAAA11100.018.3505081
GCCGTAT142400.018.13674554
CGGCCTA3500.018.061241
TCGCCGT144450.017.95157852
TCATTAA143850.017.80856560
GTCGCCG148150.017.52686351
GGTCGCC150350.017.3160750
CGCGTAG2651.8044375E-917.2282241
Produced by FastQC (version 0.11.5)