FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7289474
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166590.22853500815010797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC30450.026.3337785
CATTAAA299900.022.5932861
CGCGCAG3650.022.1221941
TCATTAA308100.021.77509160
CGCGTAG3550.021.7324982
CGTATCA321950.020.79415356
CGCGTGA3050.020.7188631
GTATCAT319300.020.60504757
CCGTATC326950.020.48685555
ATCATTA334300.020.03711159
TGTCGGC16800.019.99726730
CGCCGTA344400.019.80420553
AAAAAGG332150.019.6749770
TATCATT342000.019.5655258
AAAAGGG129050.019.55605970
CGGCGGC17550.019.54148933
GCCGTAT347450.019.519754
GTCGGCG17350.019.3633531
TCGCCGT353500.019.27446452
TTAAAAA374100.018.81437163
Produced by FastQC (version 0.11.5)