FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804724
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115610.24061735908243637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC20450.028.5954325
TGTCGTG14250.028.329191
CGCGTAG3100.026.0193182
CGCGTGA1701.3460522E-1024.7789861
TCGCGTA4350.023.4023721
AAAAGGG115400.023.08069670
CGCGAAA8100.022.535621
AGTCGTG17150.022.1060311
CGGCAAG10600.021.8569341
TGTCGGC14000.021.74738530
CGGCCTG1957.530616E-1021.6021921
CGGCGGC14600.021.57274833
CGCCAAA16650.020.8723871
CATTAAA240800.020.60927461
CTAGATC16200.020.3182545
TCGGCGG15200.020.26072132
CAAGATC30500.020.2062785
GTGGGAC16150.019.93464519
CGCGTCG4950.019.856561
GCGAGAT11950.019.644174
Produced by FastQC (version 0.11.5)