FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134783
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161610.26343230070240464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC32850.034.4313245
CATTAAA311650.029.62490761
CGTATCA312450.029.45584156
CCGTATC313650.029.28736155
TCATTAA314150.028.97458560
GTATCAT314350.028.8547857
CGCCGTA321050.028.74310553
AAAAGGG166550.028.43399670
GCCGTAT327850.028.33929854
TCGCCGT324500.028.27575552
GTCGCCG340700.027.53727151
GGTCGCC344300.027.32026750
ATCATTA333050.027.25677559
TATCATT337600.027.04491258
TGGTCGC344300.026.9238849
CTCGGTG342400.026.5724944
TCTCGGT342350.026.33105343
ATCTCGG346950.026.23452842
GTGGTCG354700.026.22324848
GATCTCG348350.026.03868141
Produced by FastQC (version 0.11.5)