FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5465621
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189770.3472066577613047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC43450.040.9450155
CGCCGTA406950.039.09214853
GGTCGCC411800.038.96209350
CTCGGTG392850.038.95309444
GTCGCCG410800.038.93839351
TCGCCGT407300.038.9207352
ATCTCGG394150.038.86048542
TCTCGGT393300.038.83770443
TGGTCGC412400.038.72721549
GATCTCG393150.038.62999341
CCGTATC404100.038.61444555
CGTATCA404750.038.55243356
GCCGTAT411050.038.49790654
GTGGTCG415900.038.3928948
GGTGGTC419700.038.04527747
AGATCTC401400.037.9319340
TCGGTGG412950.037.87059845
AAAAGGG177200.037.4679970
TAGATCG104350.037.30416
CGGTGGT425400.037.28872346