FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8002337
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166350.207876773997396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG21600.020.8059351
CGCGCAG4000.019.3105071
CGCGTAG3900.018.0051361
CGTATCA173000.017.6998156
CCGTATC173250.017.61367255
TGTCGGC20500.017.58310130
CATTAAA176300.017.56875861
TCATTAA175850.017.49320860
GTATCAT173450.017.310957
GTCGGCG21050.017.12368631
CGCCGTA181650.016.93391653
TGCGGTG14100.016.932491
TGTCGTG15850.016.8350871
GCCGTAT184850.016.65980354
CGCGAAT9700.016.650111
ATCATTA188400.016.36507259
TCGCCGT187350.016.3436952
GTCGCCG194100.015.99167351
CGGCGGC23250.015.80441633
GGTCGCC198750.015.63454950
Produced by FastQC (version 0.11.5)