FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6995575
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147880.21139077202374357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC173650.026.50118655
CGCCGTA177250.026.39692353
CGTATCA175550.026.31403756
TCGCCGT182050.025.66230452
GCCGTAT181950.025.63849354
GTCGCCG186450.025.6194151
GGTCGCC189450.025.3048150
GTATCAT180600.025.28739257
TGGTCGC193750.024.49018149
CATTAAA195750.024.10005461
TCATTAA193950.024.07092960
CTCGGTG191050.023.9023544
GTGGTCG199600.023.82548
TCTCGGT193100.023.37677443
ATCTCGG195250.023.1741242
GGTGGTC208150.022.93041847
GATCTCG197400.022.86853441
CGAGATC23750.022.8559475
ATCATTA206450.022.59670859
TATCATT206750.022.47928258
Produced by FastQC (version 0.11.5)