FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7651474
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164380.21483442275305387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG10000.020.7130171
CGGCAAG20050.019.785881
TGCGTCG8400.019.6430951
TGTCGGC23650.019.53213730
CGGGCAG6700.018.3393761
CGGCGGC24450.018.32053233
GTGGGAC26050.018.13505419
GTCGGCG25500.018.11509931
TCGGCGG25150.017.6714732
CGGGAAG57100.017.5226611
GTCCGCA35550.017.12794713
GGGTCTC34950.017.0229361
TGTCGTG16250.016.8512691
CGAGATC24150.016.8185735
TAGTCCG36750.016.6637811
AGTCCGC36650.016.61387312
CGCCAAA29000.016.5849421
CGCGAAA12850.016.3922841
GAAGCGT38900.016.01526669
CGGGGTG8150.015.9380591
Produced by FastQC (version 0.11.5)