FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144832
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114770.18677483778238363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG4900.020.7781371
TGTCGGC16750.020.26578330
TCGGCGG18400.018.63866232
CGGCGGC19450.017.63246233
GTCGGCG19250.017.45207431
GTGGGAC19700.017.23021919
CGGGAAG40850.016.1573641
GTCCGCA25150.015.72277713
CGGCAAG14400.015.6035051
TAGTCCG25400.015.56802711
GGGTCTC24700.015.16102961
AGTCCGC25800.015.05539312
CGGCAAA45900.014.7621391
CGCCAAA23650.014.5478761
CGGCAAT17700.014.4795251
GCGGCAG25400.014.32879535
CGGCCAG5450.014.171991
GAAGCGT28200.014.02511869
TAGGGTC27350.013.94762959
CGCAAAG19500.013.8631141
Produced by FastQC (version 0.11.5)