FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n02_ncm9-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n02_ncm9-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193200.21648286166140066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC75600.035.663395
AAAAGGG287700.033.4930470
CATTAAA694300.032.8260761
TCATTAA698400.032.37005660
CGTATCA716800.031.66553556
GTATCAT703400.031.66145157
CCGTATC720850.031.41480655
ATCATTA721100.031.24931759
TATCATT725800.030.92159358
GCCGTAT749350.030.6027854
CGCCGTA749300.030.58095753
TCGCCGT755650.030.25434152
GTCGCCG774450.029.84055951
ATTAAAA789900.029.57984462
GGTCGCC785350.029.53729850
TTAAAAA800150.029.40699663
TGGTCGC789200.029.18036849
CTCGGTG764950.029.11290444
TCTCGGT769150.028.79469143
GTGGTCG801800.028.75672348
Produced by FastQC (version 0.11.5)