FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5316153
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118390.2226986318866293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGA3800.034.9886473
GCGAGAT8100.030.2371044
GAGATCG44000.025.5241286
CACTCTT49100.024.7376841
ACACTCT36300.023.2392181
AAGATCG59900.022.2534186
CGAGATC25250.021.4767655
GGAGATC10750.020.5036265
ACTAGCT24500.019.43543839
CACTAGC23950.019.29609538
CCGAGAC3500.019.03144329
GCGCGAG2053.2603566E-818.7743972
CACACGT149400.018.52582619
GCACACG149650.018.51459918
CGTCTGA144150.018.47221823
ACGTCTG145300.018.34615322
GAACTCC140650.018.31534228
AGAGCAC157250.018.30926715
ACACGTC148400.018.26856420
GAGCGGG1150.001006155318.26766670
Produced by FastQC (version 0.11.5)