FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9NEW-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9NEW-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7590019
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT38000.025.2548941
GCCCACA18300.016.842641
TACGGGC7150.014.19538311
CGGGCTC8850.012.65499213
CCTATGC21200.010.89590412
GGGCTCG10800.010.69412714
ACGGGCT10950.010.22800712
CCCTACA72800.010.0017464
CACTCTT104450.09.8536482
TTACGGG11050.09.81871310
CCGATCT67150.09.79866716
CGGAGTG11100.09.77448529
CTCGAGT12250.09.71399817
GCTCGAG10850.09.67713316
TCGCGCT18900.09.4464052
GAGTGTC13050.09.38669531
CTCGCGC19850.09.3517731
GGCTCGA12500.09.23972615
CCTACAC79400.09.2144565
TCCGATC69800.09.17594515
Produced by FastQC (version 0.11.5)