FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9NEW-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9NEW-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7594059
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT39300.024.684361
TACGGGC8900.017.30293711
CGGGCTC9650.015.59546613
GCCCACA15150.014.1010621
CGGAGTG11700.014.05947729
GCTCGAG11150.013.81131516
GGGCTCG11200.013.74965614
CTTAGGG12200.012.62263461
TTACGGG12850.012.5288710
ACGGGCT11500.012.47794912
GGCTCGA12750.012.07812915
CTCGAGT12600.011.94414617
GAGTGTC13450.011.44952731
CCGGAGT15250.011.01611728
CGAGTAA15700.010.92329419
ACCGGAG14850.010.84147927
CACTCTT98850.010.2343742
CCCTACA78700.09.6965894
CCCCGGG5805.1803E-89.6549317
CGCCGGG4005.9333106E-59.6247623
Produced by FastQC (version 0.11.5)