FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6393895
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG215550.037.4752464
TGCTTGA220750.036.60832665
GCTTGAA224400.036.30920866
GCCGTCT223050.036.2622256
GAGATCG86550.035.5932276
TGCCGTC229600.035.5630955
TATGCCG230200.035.5160153
ATGCCGT231350.035.27895454
GTATGCC232550.035.2323652
CCGTCTT231650.035.0368557
CGTATGC240600.034.068151
CTCGTAT237350.034.00374249
GATCTCG232800.033.946746
CGATCAG243650.033.65626540
TCTCGTA239150.033.36730248
ATCTCGT237350.033.34018347
AGTCACG247200.033.229335
GTCACGA247350.033.19500736
TCGTATG245900.033.1772550
AGATCTC240150.032.89341745
Produced by FastQC (version 0.11.5)