FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7291443
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT48900.037.5267941
GCGTGAA37600.031.64812366
TGCGTGA31900.028.08705165
CTGCGTG45350.025.46792464
CGTGAAA47700.025.46056467
GTCGGGG8200.024.32864462
GCCGGCT24700.024.23014856
TGCCGGC59200.023.88452155
GTCTGCG26050.023.51187362
CGGCGTC38000.023.30203258
TATGCCG55850.022.37185353
GTCGGCG14400.022.36053762
GCCGGCG36750.022.28514156
GCGTGTA21100.022.22691766
CGTCTGC35300.022.20906361
ATGCCGG38500.022.1812554
CCGGCGT42950.021.757357
GCCGTCT34450.021.63950356
GCGTCTG30850.021.32846360
GGGGTCG6700.020.89498759
Produced by FastQC (version 0.11.5)