FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7067704
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA57150.035.64221666
ACACTCT44300.032.887461
TGCGTGA42900.032.8780265
CTGCGTG51100.031.02667664
CGTGAAA67650.030.73099167
TCGGCGT23750.030.0624663
GTCGGCG16550.030.02951462
GGCGTGA20000.029.92430565
CCGGCGT34750.029.71153857
CGTCGGC18150.029.6962861
CTGCTTG90150.029.54451664
GCCGTCT102950.029.47478956
CGGCGTC30050.029.1174658
GCCGGCG33000.028.74175556
TATGCCG132700.028.59014153
TGCCGGC58400.028.34694555
GAGATCG103450.028.0857126
GCTTGAA104050.027.716866
TGCCGTC134800.027.67739755
GTATGCC140700.027.61129852
Produced by FastQC (version 0.11.5)