FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934291
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT60350.043.1725961
GCTTGAA131600.027.07388966
TGCTTGA138700.025.13284365
CTGCTTG139700.024.92788364
CACTCTT154650.020.425331
CTTGAAA179650.020.41704267
GCCCACA19000.020.0902521
TCTGCTT180500.019.40955763
TGAAAAA212500.018.54548669
GAAAAAA224850.017.9004470
TTGAAAA209700.017.4912868
GCGAGAT17700.017.0079844
GAGATCG100700.016.8245536
TTCTGCT205500.016.69064162
CTTCTGC216500.016.58624861
GCCGTCT218300.016.51361856
CGCGAGA7100.016.2698383
CCGTCTT222250.016.2516257
ACTCTTT194500.016.2159422
GCGTGAA16700.015.92780166
Produced by FastQC (version 0.11.5)