FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900668
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37300.035.208841
GCGTGAA26950.027.01229566
TGCGTGA22850.022.51583365
CGTGAAA34600.022.05145667
CTGCTTG63500.021.3301664
GCTTGAA67900.021.23656866
CGGCGTG12150.020.4521564
TGCTTGA67500.020.22170365
GCCGTCT80500.020.21687356
GGCGTGA10800.019.44394565
TATGCCG107500.018.88323653
TGCCGTC100250.018.81747255
ATGCCGT97500.018.52260254
CCGTCTT91400.018.4951557
CGTCGGC9250.018.16169761
CTGCGTG30850.018.15188664
GTCGGCG9200.017.87997462
GTATGCC114450.017.85886852
TCGGCGT13500.017.62917763
GAGATCG61450.017.4317556
Produced by FastQC (version 0.11.5)