FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7289474
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT87300.043.1620941
GCTTGAA216300.031.16426766
TGCTTGA222250.029.66854765
CTGCTTG222300.029.63038464
CTTGAAA245900.027.65485867
GAAAAAA282250.025.4696970
TGAAAAA284950.024.9704269
TCTGCTT267550.024.82838263
CACTCTT163050.023.5311012
TTGAAAA291700.023.49272568
TTCTGCT282600.023.27082362
CTTCTGC293250.023.04630961
CGCGAGA10550.021.2360653
GCGAGAT23100.021.0644134
GTCTTCT324400.020.83332459
CCGTCTT330700.020.64810657
GCCGTCT330350.020.61700856
GAGATCG142400.020.6006326
CGTCTTC346650.019.84949358
TCTTCTG346450.019.51747960
Produced by FastQC (version 0.11.5)