FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804724
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT81700.052.4736941
GCGTGAA40650.032.5452866
CGTGAAA42500.032.03446267
GGCGTGA13500.029.81404165
GCTTGAA102300.028.46455866
CACTCTT167100.028.1711851
GAAAAAA172100.027.6982970
TGAAAAA166600.027.52029469
TGCGTGA32650.027.22746865
TGCTTGA97350.027.03576565
CTGCTTG99650.026.72785464
CTGCGTG36550.025.27978164
CTTGAAA120850.024.52977267
GCCCACA12450.024.4755331
GTGAAAA61250.023.71366968
GCGAGAT17400.023.1363964
CGTCGGC13050.023.06453361
TTGAAAA137100.022.20948868
TCTGCTT128350.021.73297563
TCGGCGT19000.021.55207363
Produced by FastQC (version 0.11.5)