FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134783
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA72550.039.5098266
GGCGTGA20100.036.91461665
CTGCGTG60750.036.69878464
TGCGTGA57800.035.96806765
CGTGAAA80950.035.88556367
ACACTCT53850.034.144081
CTGCTTG133900.033.71845664
GCTTGAA145050.033.56355366
TGCTTGA137000.032.9043965
GCCGTCT165400.031.35965256
GAAAAAA288750.030.75084570
CGTCGGC19200.030.62432961
TGAAAAA276900.030.04448369
GTGAAAA104450.029.95626368
CCGTCTT180800.029.88872757
TGCCGTC204800.029.85530555
TCGGCGT26650.029.8117363
ATGCCGT190550.029.44307154
TATGCCG224050.029.18034753
GTCTGCG26750.028.91525362
Produced by FastQC (version 0.11.5)