FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5465621
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG134750.040.7880636
AAGATCG185450.039.623046
CGAGATC56650.038.4372835
GCCGTCT417000.038.3309856
GATCTCG403900.038.2744146
CTGCTTG412400.038.18143564
TGCCGTC425500.038.1328155
TATGCCG428100.038.12195253
CACGATC409100.037.93369343
GTATGCC431000.037.9060552
ATGCCGT428450.037.8947654
CGTATGC431100.037.8647851
TCTCGTA411750.037.76571348
ACGATCT410150.037.70004744
CTCGTAT420300.037.69693849
ATCTCGT409950.037.6668747
CCGTCTT425450.037.6025857
TCGTATG431100.037.51568650
TGCTTGA421550.037.45231665
TCACGAT423750.037.4481842