FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8002337
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT53400.030.0359361
GCTTGAA110200.028.13916668
TGCTTGA115300.026.34811467
CTGCTTG116750.025.81103166
GCCGTCT160550.019.7068558
CCGTCTT167200.018.90212259
TCTGCTT162400.018.79271565
CTTGAAA168950.018.56132169
TGCCGTC177200.018.09217857
GCCCACA20650.017.4676361
ATGCCGT187550.017.03777156
GTCTTCT188550.016.83603561
CTTCTGC193700.016.8220663
CACTCTT141800.016.6703431
TATGCCG194850.016.50722955
CGCGAGA7450.016.4449773
TTCTGCT193850.016.0146364
GTATGCC203050.015.92678454
TTGAAAA204550.015.69021770
GAGATCG89950.015.2547966
Produced by FastQC (version 0.11.5)