FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6995575
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT43000.034.621531
GCGGCGT20350.027.5174859
GCGTGAA30350.026.86892968
CTGCGTG29250.026.68284666
TGCGTGA22950.024.70503467
GCGTGTA23950.024.69643868
GTGGGGG22900.024.14764264
TGGGGGT22100.024.07157165
CGTGTAA26050.024.04907869
GTGTAAA50350.023.9117970
GAGATCG85200.023.6672526
GCGTGGG15300.023.33253362
GGGTGTA25050.022.4942468
CGCGAGA8800.022.2776953
CGGCGTC21100.022.22672760
ATCTCGG36150.021.78348449
ATCGCGG52100.021.6979149
TGGCGGG16250.021.53772257
GGGCGTG11450.021.39664566
CGGGGGT11950.021.3800251
Produced by FastQC (version 0.11.5)