FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7651474
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT75850.040.397561
CACTCTT153850.021.623641
GCGTGAA22500.021.31064866
GCTTGAA102700.020.99272566
CTGCTTG101600.020.08322364
TGCTTGA104000.019.58611165
CGTGAAA31700.016.6715667
TGCGTGA20400.016.64179265
CTGCGTG22800.015.65755364
CTTGAAA142850.015.48443767
TGAAAAA185650.014.96870169
CGGCGTG9600.014.583014564
TCTGCTT147200.014.45620663
ACTCTTT233600.014.3410182
GAAAAAA199750.014.2975670
CGCGAGA7450.014.0962313
CGGGCTC9700.014.07185713
GCCGTCT166750.013.74782656
TACGGGC8950.013.686851511
CTTCTGC166950.013.56364561
Produced by FastQC (version 0.11.5)