FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144832
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT35650.026.130321
TACGGGC7200.017.49957511
CGGGCTC7750.016.70927213
CACTCTT73750.014.0082091
ACGGGCT9250.013.6212912
CGGAGTG10250.013.3167529
GCTCGAG9750.013.28172816
GGGCTCG10000.012.94968514
GGCTCGA10050.012.53700915
TTACGGG10150.012.41349110
CTCGAGT10900.012.20153917
CTTAGGG10700.011.77541461
CCGGAGT11750.011.31887328
CTGCTTG50100.010.75822264
ACCGGAG12600.010.555327
GAGTGTC12300.010.24365331
GCTTGAA53600.010.12102366
CGAGTAA13500.010.11086619
GCGGTAG10850.09.9999243
ATACCGG13300.09.99975725
Produced by FastQC (version 0.11.5)